But the campaign added that it was accepting the rules so that the debate could go ahead.
“We understand that Donald Trump is a risk to skip the debate altogether, as he has threatened to do previously, if we do not accede to his preferred format,” it said.
“For this reason, we accepted the full set of rules proposed by ABC, including muted microphone.”
The Trump campaign said in a statement it was “thrilled that Kamala Harris and her team of Biden campaign leftovers” have “accepted the already agreed-upon rules.”
Trump himself, however, previously indicated that he would accepted un-muted mics.
He attacked host network ABC in an interview with Fox News on Wednesday, questioning its polling and saying it was “the worst network in terms of fairness”.
He said he had only agreed to the debate “because (Ms Harris’s campaign) wouldn’t do any other network.”
Trump after hearing the acceptance: